Bolsa de Pós-doutorado em Microbiologia

Última atualização em: 05/05/22

Nível da vaga

Pós-doutorado

Título do projeto

“Microbiota Intervention Strategies Limiting Selection and Transmission of Antibiotic Resistance burden in the One Health domain (MISTAR)

Descrição da vaga

This postdoctoral position is open to candidates with strong track record of scientific publications. The successful candidate will work in the “Microbiota Intervention Strategies Limiting Selection and Transmission of Antibiotic Resistance burden in the One Health domain (MISTAR)” project, approved by JPIAMR HARISSA Call, and supported by the “International Centre for Antimicrobial Resistance Solutions – ICARS”. The purpose of MISTAR is to develop, evaluate, implement and monitor new intervention strategies to control the selection and spread of antimicrobial resistance genes (ARGs) and antimicrobial resistant bacteria (ARBs) in ´hothouses’ by modulating the intestinal microbiota of high-risk patients, “indoor microbial communities” of controlled environments and hospital sewage with impact on AMR using a One health perspective. In Brazil, our main aim is to evaluate whether the air intervention using air purifying system
could decrease dust-bound ARBs and ARGs and thus decrease this transmission route in household in the community. We also will access dogs and tutors’ microbiota to possibly track ARBs transmission among the residents. Another point is to verify whether the recent use of antibiotic in dogs could select ARBs and contribute to disseminate them to the house dust microbiota.
We will
analyze the dust microbiome by metagenome and quantify its total bacterial load by qPCR. Similar analysis will be performed to the remaining samples (nasal swabs and feces). We will isolate and identify specific bacteria of clinical interest from nasal swabs, feces, and dust to a complete characterization and comparison. In addition, using the same samples collected in the trial described above, a secondary aim is to contribute with isolates to projects in Europe, in which new approaches of microbiome modulation will be tested by our collaborators in MISTAR.

The fellowship will be provided by ICARS and is compatible
with PDJ CNPq (~R$ 4.100,00). The successful candidate will
work at the LEMiMo, in Brazil, but will keep close contact with
international research groups in this collaborative project.

Remuneração

Nenhuma

Regime / Modalidade

Presencial

Instituição

Instituto de Física, USP - Universidade De São Paulo

Localização

São Carlos, SP

Período de inscrições

De 05/05/22 até 15/05/2022 às 14:09

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Última atualização em: 05/05/22

Outros pré-requisitos

Post-doctoral research fellow 1 – PhD with expertise in microbiology and NGS (Nanopore and
Illumina) of bacterial pathogens of clinical importance (Wet-lab + Bioinformatic skills). Candidates
must show established research in areas of NGS that focus on bacteria resistome, virulome,
phylogeny, and metagenome analysis.
The successful candidate´s basic activities include to:
- Independently design and perform experiments
- Accurately document experiments and data
- Analyze and interpret data and draw relevant conclusions
- Present results at internal and external meetings/conferences
- Participate of the lab meetings every week;
– Assist in the preparation of scientific publications and reports in English;
- Contribute to writing of fellowships and grants
– Write manuals and document standard operating procedures for LEMiMo.
– Together with the other team members, overview the operation of the laboratory, including the administrative and operational tasks, like equipment maintenance and purchases;
- Work collaboratively across lab functions (including cleaning and organizing) and train other junior lab staff as needed (in special for BL-2)

Primary Responsibilities (*, experience required)
- Collect the samples of participants in their houses (Nasal swabs of dogs and tutors, houses dust,
and take the flasks of collected feces from dogs and humans) and transport the air purification
system to/from lab from/to houses for the project (field work);
- Cultivate bacteria for daily assays and prepare the -80 °C stock of isolates;
- Process different samples, extract and quantify DNA for NGS and metagenome*;
- Submit gDNA for sequencing facility for sequencing by Illumina method;
- Perform NGS library construction and sequencing (Nanopore method) *;
- Perform the NGS hybrid assemble, data analysis and bioinformatic analysis (including resistome,
virulome, typing, plasmidome, plasmid assemble, phylogeny…)*;
- Compile data for presentations, reports, and manuscripts*.
Other Important Skills
– Experience in juggling multiple tasks in parallel.
- Strong interpersonal skills; excellent oral and written communication skills
- Ability to follow safety procedures and to maintain a safe work environment (BL-2)
- Strong attention to detail, time management, excellent recordkeeping and organizational skills

Documentos necessários

Para se inscrever:

Link disponibilizado: https://a3016a3d-4b56-4872-9940-b9d220df6704.filesusr.com/ugd/d88ad6_ed41651d80604810a9198786e0314430.pdf

Email para inscrições: [email protected]

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Vaga encerrada

Bolsa de Pós-doutorado em Microbiologia

Pós-doutorado

Instituto de Física , USP

São Carlos, SP

até 15/05/2022 às 14:09:26

Presencial

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